News Center






Application of Clostridium butyricum in Animal Production -- Poultry

Category: Company News

Clostridium butyricum (Clostridium Butyricum) is a bacterium of the eubacterial kingdom in the field of bacteria. It is a Gram-positive anaerobe in the genus Clostridium (Clostridia) of the order Clostridia (Clostridiales) of the family Clostridium (Chlostridaceae) of the genus Clostridium (Clostrdium).

There are many doctoral and master theses on the application of Clostridium butyricum in animal production. Many high-quality articles have been published in high-level academic journals such as Journal of Animal Nutrition and Poultry Science. At the same time, aquaculture enterprises have also accumulated some empirical test data in concrete practice.

Application of Clostridium Butyricum in Broiler Production

The early intervention of Clostridium butyricum can reduce the inflammatory response of E. coli in the intestinal tract of broilers to a certain extent, improve the expression of antimicrobial peptide genes, and promote the production of short-chain fatty acids, thereby reducing the diarrhea caused by E. coli [1];

Clostridium butyricum can increase the content of unsaturated fatty acids in broilers and improve the antioxidant capacity of broilers [2];

Adding compound probiotics containing Clostridium butyricum into the feed can improve the growth performance of broilers, increase the digestibility of amino acids in the ileum, improve the immunity of the body, and at the same time, reduce the number of Escherichia coli in the cecum and the content of NH3 in the feces [3];

In May 2020, a large broiler one-stop enterprise research and development farm in Shandong added micro-produced Clostridium butyricum (0.5 billion cfu/kg) in Blue Valley to the diet of 8-41-day-old broilers. The test results showed that the feed-to-weight ratio of broilers in the group with Clostridium butyricum was improved by 0.05(P<0.05), the weight gain was increased by 59g(P = 0.066), the feed cost per kilogram of weight gain was reduced by 0.20 yuan, and the input-output ratio using Clostridium butyricum was greater than 10;




Application of Clostridium Butyricum in Laying Hens

The addition of Clostridium butyricum in the diet can regulate the caecal microbial flora, improve the immune function and antioxidant capacity of laying hens, and improve the laying performance and egg quality of laying hens in the later stage [4];

Clostridium butyricum supplementation can accelerate fatty acid oxidation in the liver, optimize intestinal flora and promote bile acid secretion, thereby reducing fat deposition in the liver of aging laying hens [5];

The addition of Clostridium butyricum can improve egg production, eggshell quality, immune response, antioxidant capacity and intestinal flora in the late stage of laying [6];










The addition of Clostridium butyricum from Blue Valley (0.5 billion cfu/kg) to the feed of 35-week-old laying hens improved eggshell color, beauty and cleanliness after 3 weeks of feeding. (Photo Source: Shandong Heze Biai Mu Customer Laying Chicken Farm, October 2018)




Quality control of Clostridium butyricum for animal use

Clostridium butyricum as other probiotics, different strains of its proliferation rate and the type and concentration of the product is not the same. Therefore, it is very important to screen a strain of Clostridium butyricum that is beneficial to animal health. In addition, the application in animal production also involves the tolerance of feed processing (damp heat, extrusion) and storage resistance, and the convenience of use and the matching degree of drinking water line should also be considered for drinking water products.

Through years of systematic screening, Blue Valley Zhongwei has selected a high-yield short-chain fatty acid, such as acetic acid and butyric acid, and currently the only proprietary strain of Clostridium butyricum that produces formic acid for large-scale production. The strain also has the advantages of high sporulation rate, strong stress resistance, rich enzyme production and processing resistance.

In terms of fermentation and post-treatment, Blue Valley Microsystems adopts multi-pipeline fluid production line, Siemens PCS7 process control system and unique micro-package technology to achieve accurate, efficient and stable production.

The coated Clostridium butyricum product is used in the feed, and the retention rate still reaches more than 90% after 5 minutes of conditioning at 85 ℃. The drinking water product adopts 100 instant carrier, which will not block the waterline. All products are vacuum packed with aluminum foil.


[1] Gui Guohong, Effects of early intervention of Clostridium butyricum on intestinal immune function and cecum flora structure of broilers with Escherichia coli, doctoral thesis 2020 of Guizhou University;

[2] Liao Xiu-dong, Screening of Clostridium butyricum and its effect on animal antioxidant capacity and meat meat quality, PhD thesis 2015, China Agricultural University;

  [3] Zhang ZF et al., Effects of multistrain probiotics on growth performance, apparent ileal nutrient digestibility, blood characteristics, cecal microbial shedding, and excreta odor contents in broilers. Poult Sci. 2014 Feb;

  [4] Zhan HQ et al., Effects of dietary supplementation with Clostridium butyricum on laying performance, egg quality, serum parameters, and cecal microflora of laying hens in the late phase of production. PMID: 30285187 DOI: 10.3382/ps/pey436 J Anim Sci Biotechnol. 2018 Aug 23;

[5] Wang Weiwei, Regulation of Clostridium butyricum on Intestinal Function and Lipid Metabolism of Laying Hens in Late Laying Period, 2017 of Postdoctoral Research Report of Chinese Academy of Agricultural Sciences;

  [6] H. Q. Zhan et al., Effect of dietary supplementation with Clostridium butyricumon laying performance, egg quality, serum parameters, and cecal microflora oflaying hens in the later phase of production, Poultry Science, 2018, 0: 1-8;